ABSTRACT
ABSTRACT: Goose parvovirus (GPV), also called Derzsys disease, is a viral pathogen that causes high morbidity and mortality in goslings and ducklings. In this study, we perform the molecular characterization of the GPV in Turkey. The definition of similarity to the world of GPV isolates in Turkey and construction of a phylogenetic tree was aimed. For this purpose, the presence of GPV in the liver, spleen, and intestine tissues of nine goslings with symptoms such as dysphagia, bilateral ocular swelling, eye discharge, diarrhea, and fatigue were investigated by real-time PCR method and all samples were detected as positive. According to the data obtained by molecular characterization, phylogenetic analysis of GPV has been presented in Turkey. As a result of this study, it was determined that the GPVs available in Turkey are virulent strains.
RESUMO: O parvovírus do ganso (GPV), também chamado de doença de Derzsy, é um patógeno viral que causa alta morbidade e mortalidade em gansos e patinhos. Neste estudo, objetivou-se a determinação da caracterização molecular do GPV na Turquia, a definição da similaridade com o mundo dos isolados de GPV na Turquia e a construção de uma árvore filogenética. Para tanto, a presença de GPV no fígado, baço e tecidos do intestino de nove gansos com sintomas como disfagia, edema ocular bilateral, secreção ocular, diarreia e fadiga foram investigados pelo método de PCR em tempo real e todas as amostras foram detectadas tão positivo. À luz dos dados obtidos por caracterização molecular, a análise filogenética do GPV foi apresentada na Turquia. Como resultado deste estudo, foi determinado que os GPVs disponíveis na Turquia são cepas virulentas.
ABSTRACT
Goose parvovirus (GPV), also called Derzsy's disease, is a viral pathogen that causes high morbidity and mortality in goslings and ducklings. In this study, we perform the molecular characterization of the GPV in Turkey. The definition of similarity to the world of GPV isolates in Turkey and construction of a phylogenetic tree was aimed. For this purpose, the presence of GPV in the liver, spleen, and intestine tissues of nine goslings with symptoms such as dysphagia, bilateral ocular swelling, eye discharge, diarrhea, and fatigue were investigated by real-time PCR method and all samples were detected as positive. According to the data obtained by molecular characterization, phylogenetic analysis of GPV has been presented in Turkey. As a result of this study, it was determined that the GPVs available in Turkey are virulent strains.(AU)
O parvovírus do ganso (GPV), também chamado de doença de Derzsy, é um patógeno viral que causa alta morbidade e mortalidade em gansos e patinhos. Neste estudo, objetivou-se a determinação da caracterização molecular do GPV na Turquia, a definição da similaridade com o mundo dos isolados de GPV na Turquia e a construção de uma árvore filogenética. Para tanto, a presença de GPV no fígado, baço e tecidos do intestino de nove gansos com sintomas como disfagia, edema ocular bilateral, secreção ocular, diarreia e fadiga foram investigados pelo método de PCR em tempo real e todas as amostras foram detectadas tão positivo. À luz dos dados obtidos por caracterização molecular, a análise filogenética do GPV foi apresentada na Turquia. Como resultado deste estudo, foi determinado que os GPVs disponíveis na Turquia são cepas virulentas.(AU)
Subject(s)
Animals , Phylogeny , Spleen , Parvovirus , Geese , Liver , Real-Time Polymerase Chain Reaction , Molecular BiologyABSTRACT
The aim of this study was to investigate the presence of BTV infection and possible vector species in different regions of Turkey. In the study, blood samples taken from 666 Akkaraman sheep were examined. 2000 Culicoides specimens were captured by light traps from the same provinces and 20 Culicoides spp. were identified. Blood sera samples were investigated by c-ELISA and SNT for detecting Abs to BTV. Sera samples were detected as positive 67 (10.06%) and 160 (24.02%) by SNT and ELISA, respectively. SN50 values of the 67 positive sera samples by SNT were detected between 1/2.38 and 1/200. All sheep blood samples and pools became Culicoides spp. samples were examined for BTV Ag presence by BTACE. Thirty six (5.40%) blood samples were detected as positive, but no from Culicoides pools. In the meantime, all sheep blood samples and Culicoides samples were directly investigated for BTV genome by one step RT-PCR. Fourteen (2.10%) blood samples and 7 (11.11%) Culicoides species were detected as positive. Also, the blood samples and the Culicoides samples were inoculated into Vero cell culture and passaged 5 times. Twenty nine (4.35%) blood samples cultured in Vero cell culture lines showed CPE but non CPE was observed in Culicoides samples. While 5 (17.24%) of 29 CPE positive isolates were identified as BTV by One Step RT-PCR. Total 26 samples (14 blood samples, 7 Culicoides samples and 5 supernatants) which detected BTV genome positive by One Step RT-PCR were serotyped. At the end of the study, while 23 of 26 samples were serotyped as BTV-9, two samples were serotyped as BTV-4. One sample (C. punctatus) from Culicoides was not serotyped as none of serotypes of BTV. In the present study, BTV was isolated for the first time from C. circumscriptus, C. kibunensis, and C. punctatus in Turkey.